LS media

80 ml distilled water
10 ml 10x S-base
2.5 ml 20% (w/v) glucose
0.5 ml 0.1% (w/v) L-tryptophan
0.5 ml 2% (w/v) casein
5 ml 2% (w/v) yeast extract (Difco)
0.25 ml 1 M MgCI2
0.05 ml 1 M CaCI2
autoclave all components separately
tryptophan solution: sterile filtration